I S IGAL STERIN MOLECULAR BIOLOGIST CELL CULTURE · ASSAY DEVELOPMENT

A PhD-trained molecular biologist specializing in assay development, cell-based workflows, and validation — with a career spanning academic neuroscience, synthetic immunotherapy, and preclinical industry research.

At the frontier of biological discovery

I have cultured cells for the entirety of my scientific career — my graduate school application letter began with "I love cells," and that hasn't changed. From primary human T cells and cancer cell lines at UCSF, to primary rodent neuronal and astrocyte cultures during my PhD, to testicular cell isolations at Paterna Biosciences, cell culture is the foundation of everything I do.

My expertise centers on assay development and validation. I've independently designed and standardized complex multi-parameter MACS and FACS-based isolation protocols, developed cell-based drug screening platforms in neuronal cultures, and engineered logic-gated CAR-T circuits validated through 96-well flow cytometry assays. At Paterna, this work fed directly into a GMP-compliant validation study and procedural standards now under patent filing.

I'm equally comfortable at the wet-lab/computational interface — I've built R-based automation scripts for flow cytometry and imaging data processing, worked closely with bioinformatics specialists on single-cell sequencing projects, and contributed as a scientific writer to four funded grant applications including an R01 and R21. Rigorous science and clear communication are inseparable to me.

4
Peer-Reviewed Publications
10+
Years Research Experience
4
Funded Grant Contributions
PhD
Neurobiology, U of Utah

Research career

2024–2026
Industry
Senior Scientist
Paterna Biosciences · Salt Lake City, UT
  • Standardized complex, multi-parameter MACS and FACS panels to achieve high-purity testicular cell populations for downstream therapeutic applications.
  • Authored and spearheaded novel procedural standards currently under patent filing to protect core company IP.
  • Contributed to a GMP-compliant validation study by drafting and revising technical protocols and supporting product test runs for final isolation stages.
2018–2024
Academic
Graduate Research Assistant
University of Utah · Salt Lake City, UT
  • Dissertation: "Characterizing the Neuronal Extracellular Matrix (nECM) Via Genetically Encoded HaloTag-Hapln1 Fusion."
  • Constructed and implemented novel genetic tools for high-fidelity nECM visualization in vivo/vitro.
  • Engineered a cell-based assay in neuronal cultures adaptable for drug screening.
  • Directed cross-functional research initiatives to decipher the role of nECMs in neurological disorders, including epilepsy and PTSD.
2016–2018
Academic
Research Technician
Wendell Lim Lab · UCSF · San Francisco, CA
  • Designed and tested genetic circuits in primary human T cells for adoptive immunotherapies.
  • Maintained cell lines, plasmid stocks, and managed a tissue culture room.
  • Communicated effectively to manage and prioritize multiple concurrent projects.
2015
Academic
Intern
Wendell Lim Lab · UCSF · San Francisco, CA
  • Optimized a synthetic signaling system based on Notch in mouse embryonic stem cells.
2012–2014
Clinical
Research Assistant
Stone Research Center · Vancouver General Hospital · BC, Canada
  • Aggregated and organized clinical data on treating kidney stones.

A broad toolkit

Cell Culture
Mammalian Cell Lines Primary Neuronal Culture Immune Cell Culture Testicular Cell Culture BSL2 Transfection Transduction MACS Live Imaging Assay Development
Technical Assays
ICC / IHC qPCR Western Blot Confocal Microscopy Flow Cytometry Single Cell Sorting ELISA Cytotoxicity Assays Immune Assays
Synthetic Biology
CRISPR Design Molecular Cloning Vector Design Fusion Proteins Reporter Assays AAV Production Lentivirus Production
Software & Analysis
FlowJo Imaris LASX Zeiss Blue GraphPad Prism SnapGene FIJI Ilastik Python R
Scientific Communication
Manuscript Preparation Grant Writing SOP Documentation Patent Documentation Oral Presentations Poster Presentations
In Vivo
Mouse Handling Mouse Injection Tissue Dissection

Selected publications

2024
Dynamic organization of neuronal extracellular matrix revealed by HaloTag-HAPLN1
Sterin, I., Niazi, A., Kim, J., Park, J., Park, S.
Journal of Neuroscience
2024
Microvilli regulate the release modes of alpha-tectorin to organize the domain-specific matrix architecture of the tectorial membrane
Niazi, A., Kim, J.A., Kim, D.K., Lu, D., Sterin, I., Park, J., Park, S.
Developmental Cell
2022
Neuronal Activity Reporters as Drug Screening Platforms
Sterin I, Santos AC, Park S.
Micromachines
2020
Ultraprecise T Cell recognition programs designed by transcriptionally linked multiple receptors
Williams J.Z., Allen, G.M., Shah, D., Sterin, I.S., et al.
Science

Conference presentations

2023 · Oral
Development of the Extracellular Matrix of the Brain
American Society for Matrix Biology Meeting · Salt Lake City, UT
2023 · Poster
Development of the Extracellular Matrix of the Brain
American Society for Matrix Biology Meeting · Salt Lake City, UT
2022 · Poster
Revealing Novel Architectures in the Extracellular Matrix of the Brain
Society for Neuroscience · San Diego, CA

Education & training

Doctor of Philosophy
Neurobiology
University of Utah School of Medicine
2018 – 2024
Bachelor of Science
Psychology
University of British Columbia
2010 – 2014

Let's work
together.

Open to new opportunities in research, biotech, and beyond.